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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
11/07/2022 |
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Actualizado : |
02/12/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
GARCÍA-ROCHE, M.; TALMÓN, D.; CAÑIBE, G.; ASTESSIANO, A.L.; MENDOZA, A.; QUIJANO, C.; CASSINA, A.; CARRIQUIRY, M. |
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Afiliación : |
MERCEDES GARCÍA-ROCHE V, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, 12900, Montevideo, Uruguay.; DANIEL TALMÓN, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, 12900, Montevideo, Uruguay.; GUILLERMO CAÑIBE, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, 12900, Montevideo, Uruguay.; ANA LAURA ASTESSIANO, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, 12900, Montevideo, Uruguay.; ALEJANDRO FRANCISCO MENDOZA AGUIAR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CELIA QUIJANO, Centro de Investigaciones Biomédicas (CEINBIO) and Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, 11900, Montevideo, Uruguay.; ADRIANA CASSINA, Centro de Investigaciones Biomédicas (CEINBIO) and Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, 11900, Montevideo, Uruguay.; MARIANA CARRIQUIRY, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, 12900, Montevideo, Uruguay. |
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Título : |
Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Journal of Dairy Science, July 2022, Volume 105, Issue 7, Pages 5723-5737. OPEN ACCESS. doi: https://doi.org/10.3168/jds.2021-21358 |
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ISSN : |
0022-0302 |
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DOI : |
10.3168/jds.2021-21358 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 30 September 2021, Accepted 17 March 2022, Available online 20 May 2022, Version of Record 22 June 2022. -- Corresponding author: García-Roche, M.; Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay; email:mercedesg@fagro.edu.uy -- LICENSE: This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). -- Funding: The authors thank all the staff of the Dairy Unit of the Experimental Station ?La Estanzuela? (Semillero, Uruguay) for their support in animal handling, as well as Ezequiel Jorge-Smeding (Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay) and Irene Benoit (Centro de Investigaciones Biomédicas and Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, Montevideo) for support in the analysis of blood metabolites. MGR conceived and designed laboratory experiments, performed field and laboratory experiments, analyzed the results, and wrote the original draft and revised versions of the manuscript. MC conceptualized and designed field experiment, conceived and designed laboratory experiments, supervised field and laboratory experiments and results analyses, obtained funding and administrated the project, and reviewed and edited the manuscript. CQ conceived, designed, and supervised laboratory experiments, and reviewed and edited the manuscript. AC conceived, designed, and supervised laboratory experiments. AM conceptualized and designed the field experiment and obtained funding. DT, ALA, and GC performed field and laboratory experiments. All authors reviewed the manuscript and approved the final version of the manuscript. M. García-Roche was supported by Comisión Académica de Posgrados (Montevideo, Uruguay) fellowship BDDX_2018_1#49004502. D. Talmón was supported by Agencia Nacional de Investigación e Innovación (ANII) fellowship POS_NAC_2017_1_141266. A. Cassina and C. Quijano were partially funded by grants of the Espacio Interdisciplinario-Centros, Universidad de la República (UdelaR; Montevideo, Uruguay) 2015. A. Cassina was also supported by the grant Comisión Sectorial de Investigación Científica (CSIC) grupos I+D 2014 (767). The project was funded by CSIC of the UdelaR CSIC I+D 2018 ID 103 to M. Carriquiry and C. Quijano, as well as by ANII INNOVAGRO 2018: FSA_1_2018_1_152220 to M. Carriquiry and A. Cassina. A. Mendoza received funding from the project PL_21_0_00 of the Instituto Nacional de Investigación Agropecuaria (Semillero, Uruguay). The authors have not stated any conflicts of interest. |
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Contenido : |
ABSTRACT:
The objective of this study was to assess hepatic ATP synthesis in Holstein cows of North American and New Zealand origins and the gluconeogenic pathway, one of the pathways with the highest ATP demands in the ruminant liver. Autumn-calving Holstein cows of New Zealand and North American origins were managed in a pasture-based system with supplementation of concentrate that represented approximately 33% of the predicted dry matter intake during 2017, 2018, and 2019, and hepatic biopsies were taken during mid-lactation at 174 ± 23 days in milk. Cows of both strains produced similar levels of solids-corrected milk, and no differences in body condition score were found. Plasma glucose concentrations were higher for cows of New Zealand versus North American origin. Hepatic mitochondrial function evaluated measuring oxygen consumption rates showed that mitochondrial parameters related to ATP synthesis and maximum respiratory rate were increased for cows of New Zealand compared with North American origin. However, hepatic gene expression of pyruvate carboxylase, phosphoenolpyruvate carboxykinase, and pyruvate dehydrogenase kinase was increased in North American compared with New Zealand cows. These results altogether suggest an increased activity of the tricarboxylic cycle in New Zealand cows, leading to increased ATP synthesis, whereas North American cows pull tricarboxylic cycle intermediates toward gluconeogenesis. The fact that this occurs during mid-lactation could account for the increased persistency of North American cows, especially in a pasture-based system. In addition, we observed an augmented mitochondrial density in New Zealand cows, which could be related to feed efficiency mechanisms. In sum, our results contribute to the elucidation of hepatic molecular mechanisms in dairy cows in production systems with higher inclusion of pastures. © 2022 American Dairy Science Association. MenosABSTRACT:
The objective of this study was to assess hepatic ATP synthesis in Holstein cows of North American and New Zealand origins and the gluconeogenic pathway, one of the pathways with the highest ATP demands in the ruminant liver. Autumn-calving Holstein cows of New Zealand and North American origins were managed in a pasture-based system with supplementation of concentrate that represented approximately 33% of the predicted dry matter intake during 2017, 2018, and 2019, and hepatic biopsies were taken during mid-lactation at 174 ± 23 days in milk. Cows of both strains produced similar levels of solids-corrected milk, and no differences in body condition score were found. Plasma glucose concentrations were higher for cows of New Zealand versus North American origin. Hepatic mitochondrial function evaluated measuring oxygen consumption rates showed that mitochondrial parameters related to ATP synthesis and maximum respiratory rate were increased for cows of New Zealand compared with North American origin. However, hepatic gene expression of pyruvate carboxylase, phosphoenolpyruvate carboxykinase, and pyruvate dehydrogenase kinase was increased in North American compared with New Zealand cows. These results altogether suggest an increased activity of the tricarboxylic cycle in New Zealand cows, leading to increased ATP synthesis, whereas North American cows pull tricarboxylic cycle intermediates toward gluconeogenesis. The fact that this occurs during mid-lactation could ... Presentar Todo |
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Palabras claves : |
Dairy cows; Gluconeogenesis; Grazing; Holstein-Friesian; Intermediary metabolismg. |
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Thesagro : |
VACAS LECHERAS. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16559/1/J.-Dairy-Sci.-105p.57235737-Mendoza.pdf
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Marc : |
LEADER 05614naa a2200313 a 4500
001 1063411
005 2022-12-02
008 2022 bl uuuu u00u1 u #d
022 $a0022-0302
024 7 $a10.3168/jds.2021-21358$2DOI
100 1 $aGARCÍA-ROCHE, M.
245 $aDifferential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system.$h[electronic resource]
260 $c2022
500 $aArticle history: Received 30 September 2021, Accepted 17 March 2022, Available online 20 May 2022, Version of Record 22 June 2022. -- Corresponding author: García-Roche, M.; Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay; email:mercedesg@fagro.edu.uy -- LICENSE: This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). -- Funding: The authors thank all the staff of the Dairy Unit of the Experimental Station ?La Estanzuela? (Semillero, Uruguay) for their support in animal handling, as well as Ezequiel Jorge-Smeding (Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay) and Irene Benoit (Centro de Investigaciones Biomédicas and Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, Montevideo) for support in the analysis of blood metabolites. MGR conceived and designed laboratory experiments, performed field and laboratory experiments, analyzed the results, and wrote the original draft and revised versions of the manuscript. MC conceptualized and designed field experiment, conceived and designed laboratory experiments, supervised field and laboratory experiments and results analyses, obtained funding and administrated the project, and reviewed and edited the manuscript. CQ conceived, designed, and supervised laboratory experiments, and reviewed and edited the manuscript. AC conceived, designed, and supervised laboratory experiments. AM conceptualized and designed the field experiment and obtained funding. DT, ALA, and GC performed field and laboratory experiments. All authors reviewed the manuscript and approved the final version of the manuscript. M. García-Roche was supported by Comisión Académica de Posgrados (Montevideo, Uruguay) fellowship BDDX_2018_1#49004502. D. Talmón was supported by Agencia Nacional de Investigación e Innovación (ANII) fellowship POS_NAC_2017_1_141266. A. Cassina and C. Quijano were partially funded by grants of the Espacio Interdisciplinario-Centros, Universidad de la República (UdelaR; Montevideo, Uruguay) 2015. A. Cassina was also supported by the grant Comisión Sectorial de Investigación Científica (CSIC) grupos I+D 2014 (767). The project was funded by CSIC of the UdelaR CSIC I+D 2018 ID 103 to M. Carriquiry and C. Quijano, as well as by ANII INNOVAGRO 2018: FSA_1_2018_1_152220 to M. Carriquiry and A. Cassina. A. Mendoza received funding from the project PL_21_0_00 of the Instituto Nacional de Investigación Agropecuaria (Semillero, Uruguay). The authors have not stated any conflicts of interest.
520 $aABSTRACT: The objective of this study was to assess hepatic ATP synthesis in Holstein cows of North American and New Zealand origins and the gluconeogenic pathway, one of the pathways with the highest ATP demands in the ruminant liver. Autumn-calving Holstein cows of New Zealand and North American origins were managed in a pasture-based system with supplementation of concentrate that represented approximately 33% of the predicted dry matter intake during 2017, 2018, and 2019, and hepatic biopsies were taken during mid-lactation at 174 ± 23 days in milk. Cows of both strains produced similar levels of solids-corrected milk, and no differences in body condition score were found. Plasma glucose concentrations were higher for cows of New Zealand versus North American origin. Hepatic mitochondrial function evaluated measuring oxygen consumption rates showed that mitochondrial parameters related to ATP synthesis and maximum respiratory rate were increased for cows of New Zealand compared with North American origin. However, hepatic gene expression of pyruvate carboxylase, phosphoenolpyruvate carboxykinase, and pyruvate dehydrogenase kinase was increased in North American compared with New Zealand cows. These results altogether suggest an increased activity of the tricarboxylic cycle in New Zealand cows, leading to increased ATP synthesis, whereas North American cows pull tricarboxylic cycle intermediates toward gluconeogenesis. The fact that this occurs during mid-lactation could account for the increased persistency of North American cows, especially in a pasture-based system. In addition, we observed an augmented mitochondrial density in New Zealand cows, which could be related to feed efficiency mechanisms. In sum, our results contribute to the elucidation of hepatic molecular mechanisms in dairy cows in production systems with higher inclusion of pastures. © 2022 American Dairy Science Association.
650 $aVACAS LECHERAS
653 $aDairy cows
653 $aGluconeogenesis
653 $aGrazing
653 $aHolstein-Friesian
653 $aIntermediary metabolismg
700 1 $aTALMÓN, D.
700 1 $aCAÑIBE, G.
700 1 $aASTESSIANO, A.L.
700 1 $aMENDOZA, A.
700 1 $aQUIJANO, C.
700 1 $aCASSINA, A.
700 1 $aCARRIQUIRY, M.
773 $tJournal of Dairy Science, July 2022, Volume 105, Issue 7, Pages 5723-5737. OPEN ACCESS. doi: https://doi.org/10.3168/jds.2021-21358
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INIA La Estanzuela (LE) |
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| Registros recuperados : 99 | |
| 21. |  | ALECRIM, F.; SIMÓN, C.; MARIOTTA, J.; SANTANDER, D.; MULETA, E.; LATTANZI, F.; ALVES, B.J.R.; MARCHELLI, J.P.; CIGANDA, V. Leguminosas forrajeras con taninos: promisorio potencial de mitigación de emisiones de gases de efecto invernadero en mejoramientos de campo natural. Sustentabilidad. Revista INIA Uruguay, Junio 2022, no.69, p.33-37. (Revista INIA; 69).| Tipo: Artículos en Revistas Agropecuarias |
| Biblioteca(s): INIA Las Brujas. |
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| 23. | | ROVIRA, P.J.; CARVALHO, P.C.F.; TERRA, J.A.; LATTANZI, F.; PIZZIO, R.; AYALA, W. Sustainable intensification in crop-livestock systems. In: MUREITHI, J.G.; NYANGITO, M.M.; WAMUONGO, J.W.; NJOKA, J.; NYAMBATI; E.M.; MIANO,D.; MBUKU, S.; OKOTI, M.; MARITIM. F., ed. Sustainable use of grassland and rangeland resources for improved livelihoods. Proceedings. Nairobi, (Kenia): KALRO, 2022. p. 650-654| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA Treinta y Tres. |
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| 24. | | JÁUREGUI, J.M.; OJEDA, J.J.; BERONE, G.D.; LATTANZI, F.; BAUDRACCO, J.; FARIÑA, S.; MOOT, D.J. Yield gaps of lucerne (Medicago sativa L.) in livestock systems of Argentina. Annals of Applied Biology, July 2022, Volume 181, Issue 1, pages 22-32. doi: https://doi.org/10.1111/aab.12745 Article history: Received: 24 March 2021/ Revised: 8 October 2021/ Accepted: 9 October 2021. -- Corresponding author: Jáuregui, J.M.; Facultad de Ciencias Agrarias, Universidad Nacional del Litoral, Kreder 2807, Santa Fe, Esperanza,...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
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| 25. | | PORCILE, V.; REYNO, R.; MARANGES, F.; NOLLA, F.; BECOÑA, G.; DE BRUM RODRÍGUEZ, F.; LLOVET, P.; GUTIERREZ, F.; BARAIBAR, N.; LATTANZI, F.; SOTELO, D.; ROSSI, C. ¿Conocemos los materiales forrajeros generados por la investigación uruguaya en los últimos años? Informe especial. Revista INIA Uruguay, Setiembre 2021, no.66, p. 68-77. (Revista INIA; 66).| Tipo: Artículos en Revistas Agropecuarias |
| Biblioteca(s): INIA Treinta y Tres. |
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| 26. | | GARELI, M.S.; MENDOZA, A.; BELLO, N.M.; LATTANZI, F.; FARIÑA, S.; DAVILA, J.; RAGGIO, V.; SAVIAN, J.V. Consumo y producción de leche de vacas pastoreando Dactylis glomerata durante la primavera: efecto del manejo del pastoreo y la asignación de concentrado. [abstract]. In: AUPA, Proceedings del VII Congreso Uruguayo de Producción Animal. Sección Cambio Climático y Producción Sostenible (Climate Change Section), 14 y 15 diciembre 2021. Archivos Latinoamericanos de Producción Animal, 29(Supl.1), p.3. (Archivos Latinoamericanos de Producción Animal, Vol.29, Supl.1) Corresponding author: M.S. Gareli, Instituto Nacional de Investigación Agropecuaria (INIA), Ruta 50 Km 11, La Estanzuela, Uruguay, mailto:sgareli@inia.org.uy| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 27. | | DEVINCENZI, T.; JAURENA, M.; DURANTE, M.; SAVIAN, J.V.; CIAPPESONI, G.; NAVAJAS, E.; CIGANDA, V.; LATTANZI, F.; PARUELO, J. Building the GLENCOE Platform -Grasslands LENding eConomic and ecOsystems sErvices. Frontiers in Sustainable Food Systems, June 2021, volume 516, Article 547301. OPEN ACCESS. Doi: https://doi.org/10.3389/fsufs.2021.547301 Article history: Received 31 March 2020, Accepted 26 April 2021, Published 16 June 2021.
Edited by: Iain James Gordon, Australian National University, Australia. Reviewed by: Karl Behrendt, Harper Adams University, United Kingdom;...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas; INIA Treinta y Tres. |
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| 28. | | IRISARRI, P.; IMPERIAL, J.; LATTANZI, F.; MONZA, J.; PALACIOS, J.; SANJUAN, J.; GROSSMAN, J. Editorial: Maximizing nitrogen fixation in legumes as a tool for sustainable agriculture intensification. [Editorial article]. Frontiers in Agronomy, 2021, Volume 3, Article 796717. OPEN ACCESS. doi: https://doi.org/10.3389/fagro.2021.796717 Article history: Received 17 October 2021; Accepted 26 October 2021; Published 23 November 2021.
Corresponding author: Irisarri, P.; Department of Biología Vegetal, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay;...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 29. | | CAZZULI, F.; JAURENA, M.; DURANTE, M.; CARDOZO, G.; RUGGIA, A.; BREMM, C.; LAGOMARSINO, X.; CUADRO, R.; ROVIRA, P.J.; POPPI, D.P.; LATTANZI, F. The final winter sward height of a native grassland pasture affects the quantity of the green biomass in spring. [abstract]. In: AUPA, Proceedings del VII Congreso Uruguayo de Producción Animal. Sección Cambio Climático y Producción Sostenible (Climate Change Section), 14 y 15 diciembre 2021. Archivos Latinoamericanos de Producción Animal, 29(Supl.1), p.2. (Archivos Latinoamericanos de Producción Animal, Vol.29, Supl.1) Corresponding author: F. Cazzulli, INIA Tacuarembó, Ruta 5 km 386, Tacuarembó, Uruguay. mailto:fcazzuli@inia.org.uy| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 30. | | ZANELLA P.G.; JUNIOR L.H.P.D.G; PINTO, C.E.; BALDISSERA, T.C.; WERNER, S.S.; GARAGORRY, F.C.; JAURENA, M.; LATTANZI, F.; SBRISSIA, A.F. Grazing intensity drives plant diversity but does not affect forage production in a natural grassland dominated by the tussock-forming grass Andropogon lateralis Nees. Scientific Reports, 2021, Volume 11, Issue 1, Article number 16744. Open Access. Doi: https://doi.org/10.1038/s41598-021-96208-8 Article history: Received 09 March 2021/ Accepted 06 August 2021/ Published 18 August 2021| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
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| 32. | | ABREO, E.; BEYHAUT, E.; RIVAS, F.; BARLOCCO, C.; GARAYCOCHEA, S.; LATTANZI, F.; REYNO, R. Microorganismos como bioinsumos para la agricultura: ecología, selección, formulación y uso. Revista INIA Uruguay, Setiembre 2021, no.66, p. 119-121. (Revista INIA; 66).| Tipo: Artículos en Revistas Agropecuarias |
| Biblioteca(s): INIA Treinta y Tres. |
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| 34. | | JAURENA, M.; DURANTE, M.; DEVINCENZI, T.; SAVIAN, J.V.; BENDERSKY, D.; MOOJEN, F.G.; PEREIRA, M.; SOCA, P.; QUADROS, F.L.F.; PIZZIO, R.; NABINGER, C.; CARVALHO, P.C.F.; LATTANZI, F. Native Grasslands at the Core: A New Paradigm of Intensification for the Campos of Southern South America to Increase Economic and Environmental Sustainability Front. Sustain. Food Syst., 05 March 2021, OPEN ACCESS. DOI: https://doi.org/10.3389/fsufs.2021.547834 Article history: Received: 31 March 2020, Accepted: 07 January 2021, Published: 05 March 2021. ACKNOWLEDGMENTS: The authors gratefully acknowledge Fiorella Cazulli for the
English corrections.Correspondence: Martín Jaurena...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Tacuarembó. |
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| 35. | | AMÉRICO, L.F.; DUCHINI, P.G.; SCHMITT, D-; GUZATTI, G.C.; LATTANZI, F.; FISCHER SBRISSIA, A. Nitrogen nutritional status in perennial grasses under defoliation: Do stubble height and mixed cultivation matter?. Journal of Plant Nutrition and Soil Science, Volume 184, Issue2, April 2021, Pages 208-216, April 2021. Doi: https://doi.org/10.1002/jpln.202000201 Article history: Accepted December 19, 2020;First published: 14 January 2021. Correspondence: P. G. Duchini; e-mail address: duchini_p_g@hotmail.com| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
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| 37. | | FERNANDEZ, E.; CLARIGET, J.M.; ZARZA, R.; LATTANZI, F.; BANCHERO, G.; LA MANNA, A.; CANOZZI, M.E.A. Sistemas ganaderos intensivos de la región suroeste y centro sur del país: ¿cuáles son las prácticas tecnológicas y decisiones de manejo predominantes en las empresas?. Segunda parte: Reservas forrajeras, suplementación y cultivos de cobertura. Socio-Economía. Revista INIA Uruguay, 2021, no.64, p.55-60. (Revista INIA; 64).| Tipo: Artículos en Revistas Agropecuarias |
| Biblioteca(s): INIA Las Brujas. |
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| 40. | | SAVIAN, J.V.; QUIÑONES, A.; PRAVIA, V.; CARDOZO, G.; GUIDO, A.; DEVINCENZI, T.; JAURENA, M.; ROVIRA, P.J.; LEONI, C.; CIGANDA, V.; DE BARBIERI, I.; AYALA, W.; CIAPPESONI, G.; LATTANZI, F. Un nuevo experimento de largo plazo en INIA Treinta y Tres con foco en la sostenibilidad del campo natural. Pasturas. Revista INIA Uruguay, Diciembre 2021, no.67, p.29-31. (Revista INIA; 67).| Tipo: Artículos en Revistas Agropecuarias |
| Biblioteca(s): INIA Las Brujas. |
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